Coding

Part:BBa_K2876003:Design

Designed by: Isaac Justice   Group: iGEM18_Stanford   (2018-06-22)


Lambda CL-antiAFB1-ScFv2 fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 715
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part needed a 3-Alanine linker. It was codon optimized for E. coli.

Source

BBa_K2247006. BBa_K2876001. BBa_K2876004.

References

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0 

Pei, S.a.S., D., Construction and identification of mouse Anti-AFB1 phage single-chain antibody library. Food and Biological Engineering College, 2010.


Li, X., Li, P. and Zhang, Q., Molecular analysis of monoclonal antibodies against aflatoxins: a prediction of functional amino acid which influences antibody affinity. Chinese Academy of Agricultural Sciences, 2011.